P7777
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P7777
Sigma
Poly(ethylene glycol)
Hybri-Max™, waxy solid, mol wt 1,300-1,600, hybridoma tested
| Synonym: | PEG |
| CAS Number: | 25322-68-3 |
| Linear Formula: | H(OCH2CH2)nOH |
| MDL number: | MFCD00081839 |
| PubChem Substance ID: | 24898877 |
Description
| Application | Recommended for cell fusion. |
| Reconstitution | Melt the PEG at 45 °C or higher in a waterbath. Dilute to 30-50% using sterile, pre-warmed serum-free medium (e.g., for a 50% solution add 5 mL to the 5 g vial of PEG). The pH of the solution should be slightly alkaline (pink, not orange or purple). Adjustment of the pH with sterile NaOH may be required. Store solution below 0 °C. Prolonged storage or repeated freezing and thawing is not recommended. |
| Legal Information | Hybri-Max is a trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co. |
Properties
| grade | Hybri-Max™ |
| vapor density | >1 (vs air) |
| vapor pressure | <0.01 mmHg ( 20 °C) |
| sterility | autoclaved |
| form | waxy solid |
| autoignition temp. | 581 °F |
| mol wt | mol wt 1,300-1,600 |
| total impurities | Endotoxin, tested |
| suitability | hybridoma tested |
Safety
| Personal Protective Equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
| WGK Germany | 1 |
| RTECS | TQ3500000 |
| Flash Point(F) | 444.2 °F |
| Flash Point(C) | 229 °C |
References
| Merck | Merck 13,7651 |
| Fieser | Fieser 9,366 / Fieser 9,376 / Fieser 12,399 |
| reference | Structure Index 1, 498:C:4 |
